Link between exposure of fish (

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Identifiant documentaire 10-2004040
Identifiant OAI oai:edpsciences.org:dkey/10.1051/alr:2004040
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Auteur(s): Hélène Budzinski,Olivier Mazéas,Jacek Tronczynski,Yves Désaunay,Gilles Bocquené,Guy Claireaux
Mots clés PAH metabolites Fish Gas chromatography-mass spectrometry
Date de publication 15/10/2004
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Source https://doi.org/10.1051/alr:2004040
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An analytical method consisting in enzymatic deconjugation, solid phase extraction and purification, and gas chromatography/mass spectrometry analysis after derivatization was used in this study to quantify Polycyclic Aromatic Hydrocarbon (PAH) metabolites in the bile of fish. The method has been applied in a laboratory experiment studying the fate of pyrene in basin containing soles. This study has allowed the identification of 1-hydroxypyrene as the single metabolite in bile after enzymatic deconjugation. In a second time, 1-hydroxypyrene has been used as a biomarker of exposure in the case of the “Erika” oil spill. This biomonitoring was successful in demonstrating the exposure of juvenile soles to PAHs present in the “Erika” fuel oil.
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