Isolement et caracterisation d'une proteine membranaire ovocytaire capable de fixer specifiquement la vitellogenine

After solubilization of ovocyte-membranes from the crayfish Orconectes limosus , a component with a molecular weight of 30 kDa and capable to fix vitellogenin specifically, could be characterized by SDS-PAGE and by electroblotting. Because this isolated component is sensible to the digestion of proteolytic enzymes such as pronase, it is assumed that it is a protein. A polyclonal antiserum is produced against this component and its tissue-specificity is verified by ELISA.

Auteurs du document :

Jugan, P, Vanherp, F

Obtenir le document :

Actes de colloques. Ifremer. Brest [ACTES COLLOQ. IFREMER.]. 1988

Mots clés :

Orconectes limosus, Malacostraca, Oogenesis, Biochemical analysis, Cell membranes

Thème (issu du Text Mining) :

BIOCHIMIE - CHIMIE

Date :

1987-06

Format :

text/xml