Short-term storage and cryopreservation of turbot

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Éditeur(s) EDP Sciences
Identifiant documentaire 10-1997028
Identifiant OAI oai:edpsciences.org:dkey/10.1051/alr:1997028
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Auteur(s): Olvido Chereguini,Rosa Maria Cal,Catherine Dreanno,Bénédicte Ogier de Baulny,Marc Suquet,Gérard Maisse
Mots clés Turbot sperm storage cryopreservation
Date de publication 15/07/1997
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Source https://doi.org/10.1051/alr:1997028
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Description
Short-term storage over several days as well as cryopreservation of turbot (Scophthalmus maximus) sperm were studied. Two extenders, Ringer 200 and artificial seminal liquid (ASL2), are suggested for semen collection in order to avoid the deleterious effect of urine contamination, and for the purpose of short-term storage between 0 and 15 °C. Oxygen atmosphere is not suitable for turbot sperm storage. Turbot spermatozoa undergo cryopreservation with a high rate of success especially in a sucrose solution with 10% dimethyl sulfoxide (DMSO) and 10% egg yolk.
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