Polyploidy in the Manila clam,

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Éditeur(s) EDP Sciences
Identifiant documentaire 10-1990010
Identifiant OAI oai:edpsciences.org:dkey/10.1051/alr:1990010
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Auteur(s): Alain Diter,Christian Dufy
Mots clés Cytochalasin B tetraploidy bivalve Cytochalasine B tétraploïdie bivalve
Date de publication 15/04/1990
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Source https://doi.org/10.1051/alr:1990010
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Description
Tetraploidy was induced in embryos of Ruditapes philippinarum by treating the eggs with cytochalasin B (CB). Two treatment times were found to be effective in inducing tetraploidy: 0 to 10 minutes after insemination (before formation of the first polar body) and 45 minutes after insemination (just before the first cleavage). Treatments, started 5 and 45 minutes after insemination, produced $64.4\pm7.8{\%}$ and $28.3\pm2.3{\%}$ tetraploid embryos, respectively, after 6 hours (on the average over 3 experiments). However, no tetraploids were detected in 4-month-old spat. Sperm entry delayed by the CB could be involved in induction of tetraploidy by early treatment.

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