LACK OF SPECIFICITY OF THE MOLECULAR DIAGNOSTIC METHOD FOR IDENTIFICATION OF APHANOMYCES ASTACI

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Éditeur(s) EDP Sciences
Identifiant documentaire 11-2007002
Identifiant OAI oai:edpsciences.org:dkey/10.1051/kmae:2007002
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Auteur(s): I. BALLESTEROS,M. P. MARTÍN,L. CERENIUS,K. SÖDERHÄLL,M. T. TELLERÍA,J. DIÉGUEZ-URIBEONDO
Mots clés Oomycetes crayfish plague ITS crayfish Conservation invasive species Oomycètes peste de l’écrevisse ITS écrevisse conservation espèces invasives
Date de publication 01/04/2008
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Source https://doi.org/10.1051/kmae:2007002
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A recent PCR-test developed for identification of Aphanomyces astaci, the organism responsible for crayfish plague, provided false positives for Aphanomyces frigidophilus, Aphanomyces repetans, and some Saprolegnia spp. Real-time PCR showed that with the designed primers, A. astaci and A. frigidophilus cannot be distinguished. The results of this study show that this particular crayfish plague PCR-test ought to be improved and that molecular-based techniques need to be contrasted to histological evidences and disease history.

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