Document généré le 26/05/2026 depuis l'adresse: https://www.documentation.eauetbiodiversite.fr/fr/notice/Evaluation-d-une-suite-de-bio-essais-pour-la-detection-et-l-etude-de-composes-lipophiles-de-micro-organismes-marins-issus-de-mollusques-bivalves-et-de-leur-environnement
Évaluation d’une suite de bio-essais pour la détection et l’étude de composés lipophiles de micro-organismes marins issus de mollusques bivalves et de leur environnement
Titre alternatif
Producteur
Contributeur(s)
Éditeur(s)
Université de Nantes. Facultés des Sciences Pharmaceutiques.Ecole doctorale végétal, environnement, nutrition, agroalimentaire, mer
Identifiant documentaire
9-29426
Identifiant OAI
oai:archimer.ifremer.fr:29426
Auteur(s):
Geiger, Marie
Mots clés
cytotoxicité
larves de diptères
activité antibactérienne
toxines lipophiles
micro-algues
dinoflagellés
micromycètes
Penicillium
acide okadaïque
azaspiracide
pinnatoxine G
Vulcanodinium rugosum
Prorocentrum lima
Azadinium spinosum
Beauveria brongniartii
cytotoxicity
diptera larvae
antibacterial activity
lipophilic toxins
microalgae
dinoflagellates
marine fungi
Penicillium
okadaic acid
azaspiracid
pinnatoxin G
Vulcanodinium rugosum
Azadinium spinosum
Prorocentrum lima
Beauveria brongniartii
Date de publication
25/09/2013
Date de création
Date de modification
Date d'acceptation du document
Date de dépôt légal
Langue
fre
Thème
Type de ressource
Source
Droits de réutilisation
2013 Université de Nantes
Région
Département
Commune
Description
The context of this study was the assessment of the safety of edible bivalve molluscs. Bivalve can indeed accumulate high concentrations of phycotoxins into their hepatopancreas, potentially leading to human intoxication outbreaks after consumption of such contaminated bivalves. In order to ensure the protection of shellfish consumers, regulated toxins are monitored using physico-chemical techniques. In parallel, emerging toxins, that can be putatively produced by microalgae or marine fungi, are monitored using the intraperitoneal mouse bioassay. However, this bioassay suffers ethical and methodological drawbacks.
Thus, the objective of this work was to assess the ability of a bioassay suite to detect lipophilic toxins. This suite was composed of three types of assays: cytotoxicity on KB cells, a bioassay on diptera larvae and antibacterial activity on marine bacteria. These bioassays were first adapted to routine protocols, subsequently their detection perimeter was evaluated at different levels of matrix complexity: known or emerging pure toxin, crude extract of microorganisms producing the toxin, and spiked shellfish matrices. These three bioassays exhibited a complementary scope of detection, and an approach for their implementation was proposed.
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